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A FAP/Protein G fusion reagent targets a surface marker on live cells. A: Flow-cytometry histograms of live U937 cells HA-tagged at the surface, and labeled with anti-HA antibody and (MG-FAP)-PrG affinity reagent. Each of the panels represents a minimum of 10K live cell events. B: Micrographs of same cells as (A) acquired with a 40_ objective. All data were measured in presence of 100 nM MG-2p fluorogen.
