Table 1.

The location of N/C terminal and signature residues, and potential transmembrane helix number (THN)

Name	N-Ter	G 1 /S	G 2	G 3	G 4	C 1	C 2	K	R	THN	C-Ter
AtHKT1;1	inside	H	H	o	H	i	i	H	i	12	inside
ThHKT1;1	inside	H	H	o	H	O	o	H	i	10	inside
OsHKT1;1	outside	I	H	o	i	i	i	i	H	10	outside
EcHKT1;1	inside	H	H	o	H	O	o	H	i	12	inside
PtHKT1;1	outside	H	H	i	H	o	o	H	i	11	inside
McHKt1;1	inside	H	H	i	H	o	o	H	i	12	inside
McHKT1;2	inside	I	H	o	H	O	o	H	i	10	inside
OsHKT1;3	outside	i	H	o	H	i	i	H	o	10	outside
TmHKT1;4A1	inside	o	H	i	H	o	o	H	i	10	inside
TmHKT1;4A2	inside	o	H	i	H	o	o	H	i	10	inside
SbHKT1;4	outside	o	H	i	H	o	o	H	i	11	inside
OsHKT1;4	outside	H	O	i	H	o	o	H	i	11	inside
TmHKT1;5	outside	H	H	i	H	o	o	H	i	13	inside
TaHKT1;5	outside	H	O	o	H	O	o	H	i	9	inside
TtHKT1;5B2	outside	H	O	o	H	O	o	H	i	9	inside
HvHKT1;5	inside	o	H	i	o	o	o	o	H	10	inside
ZmHKT1;5	inside	H	H	i	o	o	o	o	H	10	inside
OsHKT1;5	outside	H	O	i	O	o	O	O	O	10	outside
OsHKT2;1	outside	H	H	o	o	i	i	H	o	12	outside
PhaHKT2;1e	outside	H	H	o	o	O	O	o	H	11	inside
TaHKT2;1	inside	H	O	i	o	o	o	o	H	12	inside
HvHKT2;1	outside	H	H	i	o	o	o	o	H	11	inside
PutHKT2;1	inside	H	H	i	o	o	o	o	H	12	inside
PhaHKT2;1n	outside	H	H	o	o	O	O	o	H	11	inside
OsHKT2;2	outside	H	O	o	O	O	O	O	O	8	outside
SbHKT2;3	outside	H	O	o	H	O	o	H	i	9	inside
OsHKT2;3	outside	H	O	i	H	o	o	H	i	11	inside
OsHKT2;4	inside	H	H	i	H	o	o	H	i	12	inside
PpHKT3;1	outside	H	H	H	O	O	O	O	O	8	outside
SmHKT3;1	inside	o	O	o	H	H	i	H	o	9	outside
SmHKT3;2	inside	o	H	i	H	o	o	H	i	10	inside
SmHKT3;4	inside	H	H	o	H	o	o	H	i	11	outside
SmHKT3;5	outside	H	H	i	H	i	i	H	o	11	inside

Abbreviations represent: H, membrane helix; I, inside loop; i, inside helix tail; O, outside loop; o, outside helix tail. G: glycine (Gly); S: serine (Ser); C: cysteine (Cys); K: lysine (Lys); R: arginine (Arg).