Table 1.
Molecular function enrichment analysis of early, mid and late genes. Analysis was performed using the STRING database. Gene ontology biological processes of the top 15 enriched functions are shown (P-value, without correction). MAP, mitogen-activated protein; MAPK, mitogen-activated protein kinase
| Term | Number of genes | P-value (not corrected) |
|---|---|---|
| Early responsive genes | ||
| Negative regulation of cellular process | 26 | 4.63 × 10−11 |
| Regulation of transcription from RNA polymerase II promoter | 18 | 1.04 × 10−10 |
| Negative regulation of biological process | 26 | 3.07 × 10−10 |
| Blood vessel development | 12 | 3.45 × 10−10 |
| Vasculature development | 12 | 5.96 × 10−10 |
| Tissue development | 17 | 9.74 × 10−10 |
| Anatomical structure formation involved in morphogenesis | 14 | 1.28 × 10−9 |
| Regulation of signaling | 21 | 1.57 × 10−9 |
| Transcription from RNA polymerase II promoter | 12 | 1.79 × 10−9 |
| Response to organic substance | 20 | 4.72 × 10−9 |
| Cardiovascular system development | 13 | 6.15 × 10−9 |
| Circulatory system development | 13 | 6.15 × 10−9 |
| Negative regulation of macromolecule metabolic process | 17 | 9.27 × 10−9 |
| Blood vessel morphogenesis | 10 | 1.08 × 10−8 |
| Negative regulation of cellular macromolecule biosynthetic process | 14 | 2.22 × 10−8 |
| Mid responsive genes | ||
| Negative regulation of intracellular protein kinase cascade | 5 | 2.30 × 10−6 |
| Negative regulation of response to stimulus | 9 | 3.96 × 10−6 |
| Negative regulation of signal transduction | 8 | 7.80 × 10−6 |
| Negative regulation of MAPK cascade | 4 | 9.98 × 10−6 |
| Negative regulation of signaling | 8 | 1.34 × 10−5 |
| Negative regulation of cell communication | 8 | 1.42 × 10−5 |
| Regulation of MAPK cascade | 6 | 1.97 × 10−5 |
| Regulation of intracellular protein kinase cascade | 7 | 5.86 × 10−5 |
| Regulation of signaling | 12 | 6.43 × 10−5 |
| Regulation of signal transduction | 11 | 8.99 × 10−5 |
| Tissue development | 9 | 1.17 × 10−4 |
| Regulation of cell communication | 10 | 1.60 × 10−4 |
| Endoderm development | 3 | 1.73 × 10−4 |
| Negative regulation of MAP kinase activity | 3 | 1.83 × 10−4 |
| Regulation of response to stimulus | 12 | 2.69 × 10−4 |
| Late responsive genes | ||
| MAPK cascade | 7 | 2.12 × 10−6 |
| Protein deamination | 2 | 1.10 × 10−5 |
| Negative regulation of immune system process | 6 | 1.19 × 10−5 |
| Epidermis development | 7 | 1.22 × 10−5 |
| Regulation of anatomical structure morphogenesis | 9 | 2.83 × 10−5 |
| Cell surface receptor signaling pathway | 17 | 3.09 × 10−5 |
| Positive regulation of norepinephrine secretion | 2 | 3.31 × 10−5 |
| Regulation of immune system process | 11 | 3.51 × 10−5 |
| Positive regulation of cell migration | 6 | 4.28 × 10−5 |
| Cell migration | 9 | 4.39 × 10−5 |
| Positive regulation of cell motility | 6 | 4.66 × 10−5 |
| Positive regulation of locomotion | 6 | 5.52 × 10−5 |
| Positive regulation of multicellular organismal process | 8 | 5.76 × 10−5 |
| Regulation of body fluid levels | 9 | 5.82 × 10−5 |
| Positive regulation of cellular component movement | 6 | 5.83 × 10−5 |