DUSP3-deficient platelets exhibit impaired GPVI- and CLEC-2-mediated platelet aggregation. (A-E) Washed platelets prepared from WT or Dusp3-KO mice were stimulated with collagen (0.5 and 1 μg/mL) (A), CRP (0.1, 0.3, and 1 μg/mL) (B), CVX (5, 10, and 100 ng/mL) (C), rhodocytin (2.5, 5, and 10 nM) (D), or thromboxane A2 analog U46619 (1 μM), thrombin (0.05 U/mL), or ADP (20 μM) (E). Representative platelet aggregation curves of three individual experiments are shown.