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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Circ Arrhythm Electrophysiol. 2014 Dec 9;8(1):165–173. doi: 10.1161/CIRCEP.114.001789

Figure 7.

Figure 7

Representative, continuous monobromobimane (mBB) fluorescence image of SERCA2a free thiols (inverse of SERCA2a-SOx) and SERCA2a protein (110 KD) from a sham, untreated (MI) and ALLO treated MI heart (MI+ALLO). DTT (fully reduced) and NEM (fully oxidized) were used to normalize fluorescence intensities. Greater fluorescence intensity indicates more free (less oxidized) thiols. Pooled data (bottom) demonstrate that treatment with ALLO (n=5) significantly increases the number of SERCA2a free thiols (decreases oxidation) compared to MI (n=5, p < 0.05) but not compared to sham (n=4, p = ns).