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. 2015 Feb 5;8:78. doi: 10.1186/s13071-015-0689-5

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© Tang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blot analysis of Ts-CLP and its recombinant protein. (A) The antigenicity of rTs-CLP by Western blot analysis. M: protein standard; 1: sera of mice at 35 days p.i.; 2: sera of pigs at 26 days p.i.; 3: sera of rabbits immunised against rTs-CLP; 4: preimmune sera of pigs. (B) Western blot analysis of ES products using the sera of rTs-CLP immunised rabbits. M: protein standard; 1: purified rTs-CLP; 2–5: ES products of NBL, Ad5, ML, L6h.