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. 2015 Feb 5;8:78. doi: 10.1186/s13071-015-0689-5

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© Tang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Real-time quantitative PCR analysis of Ts-clp. After reverse transcription, the cDNA of ML, L6h, L24h, Ad2, Ad3, Ad5, and NBL was used as a template for real-time quantitative PCR using SYBR Green. Each reaction was performed in triplicate on the plate, and the experiment was repeated three times. All the fold changes were relative to the NBL stage. ^*Data are significantly different from NBL (P < 0.05).