Figure 1.

Effect of heparanase overexpression on Panc1 carcinoma. A) Heparanase overexpression in Panc1-Hpa cells was confirmed by enzymatic activity assay. Inset: heparanase mRNA expression in Panc1-Vo and Panc1-Hpa cells, measured by real-time reverse transcription–polymerase chain reaction and normalized to actin mRNA. Data shown are representative of three independent experiments. B) Changes in primary tumor growth in SCID mice injected with Panc1-Vo cells (gray line) and Panc1-Hpa cells (black line), n = 5, *two-sided Mann–Whitney test P = .05. C) In vitro proliferation rate of Panc1-Vo cells (gray line) and Panc1-Hpa cells (black line) assessed by MTS assay. Each data point shows the mean of pentaplicate cultures. Error bars represent standard deviation.