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. 2014 Aug 4;142(1):6–20. doi: 10.1093/toxsci/kfu158

FIG. 6.

FIG. 6.

Western blot analysis of AMPK-TSC signaling proteins from WT and KD mice treated with or without paraquat (45 mg/kg, i.p.) or vehicle for 48 h. (A) Representative gel blots depicting pan and phosphorylated AMPK, TSC2, and GAPDH (loading control) using specific antibodies; (B) AMPKα; (C) phospho-AMPKα (pAMPK, Thr172); (D) pAMPKα-to-AMPKα ratio; (E) pan TSC2; (F) phospho-TSC2 (pTSC2, Ser939); (G) pTSC2-to-TSC2 ratio. Mean ± SEM, n = 4–5; *p < 0.05 versus WT group, #p < 0.05 versus WT-paraquat group. Gel density of all groups was normalized with the respective gel density of WT group.