FIG. 7.

Western blot analysis of mTORC1-related autophage signaling proteins from WT and KD mice treated with or without paraquat (45 mg/kg, i.p.) or vehicle for 48 h. (A) Representative gel blots depicting mTOR, phospho-mTOR (pmTOR, Ser²⁴⁴⁸), S6K, phospho-S6K (pS6K, Ser⁷⁹²), ULK1, phosphoULK1 (pULK1, Ser⁷⁵⁷ and pULK1, Ser⁷⁷⁷), and α-tubulin (loading control) using specific antibodies; (B) pan mTOR; (C) pmTOR; (D) pmTOR-to-mTOR ratio; (E) pan S6K; (F) pS6K; (G) pS6K-to-S6K ratio; (H) pan ULK1; (I) pULK1 Ser⁷⁵⁷; (J) pULK1⁷⁵⁷-to-ULK1 ratio; (K) pULK1 Ser⁷⁷⁷; (L) pULK1⁷⁷⁷-to-ULK1 ratio. Mean ± SEM, n = 4–5, *p < 0.05 versus WT group, ^#p < 0.05 versus WT-paraquat group. Gel density of all groups was normalized with the respective gel density of WT group.