Figure 2.

Selection strategy of zinc-finger nuclease–transfected Plasmodium vivax parasites. Charts show courses of parasitemia for 4 different Saimiri boliviensis monkeys (animals 3848, 3073, 3904, and 4909) inoculated with electroporated parasites. The arrows indicate the time points at which the doses of 4 mg/kg pyrimethamine (Pyr) were administered. In the S. boliviensis 3848 and 3073, drug selection was started 72 hours after inoculation. Animals 3904 and 4909 were treated with Pyr as soon as parasites were detected in their blood. Time points at which blood specimens were collected for polymerase chain reaction analysis are indicated by black stars.