Table 4.
Association between expression levels of miR-885-5p and CRC prognosis and metastasis prediction in the matched CRC tissue and serum cohorts (175 FFPE pCRC tissues and 169 sera)
| Characteristics | Univariate | Multivariable | ||
|---|---|---|---|---|
| HR (95% CI) | P | HR (95% CI) | P | |
| Association between miRNA expression and CRC prognosis (Cox proportional hazards model on overall survival) | ||||
| Age (>68 y vs ≤68 y) | 0.76 (0.43 to 1.35) | .3533 | 1.17 (0.50 to 2.76) | .7213 |
| Sex (male vs female) | 1.09 (0.60 to 1.96) | .7875 | 1.40 (0.59 to 3.28) | .4461 |
| Tumor size (>40mm vs ≤40mm) | 2.35 (1.27 to 4.35) | .0066 | 0.78 (0.29 to 2.10) | .6302 |
| Lymph node metastasis (present vs absent) | 17.05 (6.13 to 47.44) | <.0001 | 7.80 (1.55 to 39.34) | .0133 |
| Distant metastasis (present vs absent) | 35.93 (14.78 to 87.42) | <.0001 | 15.09 (4.74 to 48.10) | <.0001 |
| CEA (>5ng/mL vs ≤5ng/mL) | 5.14 (2.29 to 11.56) | .0001 | 0.87 (0.26 to 2.85) | .8125 |
| Tissue miR-885-5p (high vs low) | 0.95 (0.48 to 1.90) | .895 | 0.87 (0.33 to 2.30) | .7793 |
| Serum miR-885-5p (high vs low) | 3.94 (1.91 to 8.11) | .0002 | 2.87 (1.10 to 7.51) | .0323 |
| Association between miRNA expression and CRC lymph node metastasis (logistic regression model) | ||||
| Age (>68 y vs ≤68 y) | 0.86 (0.49 to 1.50) | .5922 | 1.48 (0.65 to 3.36) | .3518 |
| Sex (male vs female) | 0.81 (0.46 to 1.43) | .4736 | 0.54 (0.25 to 1.19) | .1272 |
| Tumor size (>40mm vs ≤40mm) | 2.26 (1.28 to 4.01) | .0052 | 1.89 (0.82 to 4.32) | .1336 |
| Pathology (poor diff. vs diff.) | 2.14 (0.79 to 5.78) | .1322 | 1.65 (0.41 to 6.65) | .4817 |
| Venous Invasion (present vs absent) | 3.99 (2.21 to 7.22) | <.0001 | 2.03 (0.91 to 4.52) | .0835 |
| CEA (>5ng/mL vs ≤5ng/mL) | 3.60 (1.90 to 6.84) | .0001 | 2.12 (0.92 to 4.86) | .0767 |
| Tissue miR-885-5p (high vs low) | 0.59 (0.31 to 1.16) | .1269 | 0.47 (0.20 to 1.09) | .0788 |
| Serum miR-885-5p (high vs low) | 2.08 (1.11 to 3.89) | .0226 | 3.03 (1.28 to 7.18) | .0116 |
| Association between miRNA expression and CRC distant metastasis (logistic regression model) | ||||
| Tumor size (>40mm vs ≤40mm) | 3.32 (1.59 to 6.93) | .0014 | 1.81 (0.52 to 6.37) | .3545 |
| Lymph node metastasis (present vs absent) | 33.17 (9.78 to 112.47) | <.0001 | 34.73 (4.32 to 279.04) | .0008 |
| Venous Invasion (present vs absent) | 5.78 (2.71 to 12.31) | <.0001 | 4.82 (1.36 to 17.03) | .0146 |
| Tissue miR-885-5p (high vs low) | 0.64 (0.28 to 1.47) | .2941 | 0.57 (0.16 to 2.00) | .3824 |
| Serum miR-885-5p (high vs low) | 3.42 (1.45 to 8.06) | .005 | 3.12 (0.97 to 10.03) | .0456 |
* All statistical tests were two-sided. CI = confidence interval; CRC = colorectal cancer; HR = hazard ratio; miRNA = microRNA; OR = odds ratio; pCRC = primary CRC.