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The enzyme efficiency, represented as V max /K m for LDH in partial purified tumor and normal breast tissues
| Tumor | Normal | |
|---|---|---|
| Vmax/Km with lactate (mU/mg/mM) | 28.92 ± 1.3* | 56.1 ± 2.4 |
| Vmax/Km with NAD+ (mU/mg/mM) | 1294.94 ± 6.7* | 2474 ± 8.1 |
Assays were conducted at 25°C and data are presented as means ± SEM, n = 3 independent determinations on each of 17 tumor and normal samples. *Significant difference in each row at p < 0.05.
