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. 2015 Feb 15;13:52. doi: 10.1186/s12957-015-0465-1

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© Yu et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SDF-1 secretion and SDF-1 and CXCR4 expression. (A) The SDF-1 concentration in the BMSC supernatant as detected by ELISA. BMSCs had significantly higher levels of SDF-1 compared to MG-63 and OS732 cells. (B) The RT-PCR analysis of SDF-1 mRNA expression in osteosarcoma cell lines and BMSCs. (C) The RT-PCR analysis of CXCR4 mRNA expression in osteosarcoma cell lines and BMSCs. (*P < 0.01 compared to the BMSCs).