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. 2015 Feb 18;11(2):e1004995. doi: 10.1371/journal.pgen.1004995

Fig 6. Cell-autonomous accumulation of MG-AGEs in FASN mutant FB cells.

Fig 6

Immunodetection of MG-AGEs (B,E,H,K,N) and DAPI staining (C,F,I,L,O,O’) in FB from feeding L3 larvae containing MARCM clones labeled with GFP (A,D,G,J,M). (A-F) Accumulation of MG-AGEs in FASN Δ24-23 MARCN clones of L3 larvae fed either a LCD (A-C) or a 20%-SSD (D-F). (G-L) Suppression of MG-AGE accumulation in FASN Δ24-23 MARCN clones of L3 larvae fed a 20%-SSD by either PFK1-RNAi (G-I) or UAS-glo1 (J-L). (M-O) Accumulation of MG-AGEs and extreme nuclear size reduction in FASN Δ24-23 MARCN clones expressing glo1-RNAi of L3 larvae fed a LCD. (O’) is an orthogonal section of (O). Scale bars: 20μm. For each condition, at least 10 larvae were dissected.