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. 2015 Feb 18;10(2):e0118168. doi: 10.1371/journal.pone.0118168

Fig 4. The GSI-I treatment inhibits hUC-MSC activities in suppressing Th1 lymphocyte proliferation and IDO1 expression.

Fig 4

A. Flow cytometry assay shows that co-culturing of hUC-MSC with PBMC in 1:5 ratio significantly inhibits Th1 lymphocyte proliferation. However, the treatment with 2.5 μM GSI-I significantly inhibits the effect of hUC-MSCs on Th1 proliferation. A-1: bar figure; A-2: original spectrogram of a representative Th1 lymphocyte analysis. Th1 lymphocytes are CD8-/IFN-g+ cells as circled in the spectrogram. B. The GSI-I treatment reduces the IFN-γ-induced IDO1 expression. Both semi-quantitative RT-PCR (B-1) and Western blotting (B-2) show that, while 2ng/mL IFN- γ induces IDO1 expression in hUC-MSCs, greater than 2.5 M GSI-I dramatically reduces the IDO1 expression.