Table 3.

Primer sequences for MSP

Gene	Primer sequence
INK4/p16 U FORWARD	5’-TTA TTA GAG GGT GGG GTG GAT TGT-3’
INK4/p16 U REVERSE	5’-CAA CCC CAA ACC ACA ACC ATA A-3’
INK4/p16 M FORWARD	5’-TTA TTA GAG GGT GGG GCG GAT CGC-3’
INK4/p16 M REVERSE	5’-GAC CCC GAA CCG CGA CCG TAA-3’
TP53 U FORWARD	5’-TTT TTT AGG TAG TTT TTG GTT TTG T-3’
TP53 U REVERSE	5’-ACC AAA CCT CTC AAA TTA CAA CAA T-3’
TP53 M FORWARD	5’-ATT TTT TTA GGT AGT TTT CGG TTT C-3’
TP53 M REVERSE	5’-GAA CCT CTC AAA TTA CGA CGA T-3’

Primers for INK4/p16 unmethylated (U) amplified a region of 151 bp, primers for INK4/p16 methyled (M) amplified a region of 150 bp; primers for TP53 unmethylated amplified a region of 134 bp, primers for TP53 methylated amplified a region of 133 bp.