Figure 4.

Adipocyte differentiation of chin- and knee-ASCs. ASCs were induced to undergo differentiation for 10 days. (a) Cells were stained with Oil Red O for lipid droplets. (b) Cells were harvested and analyzed for their triglyceride content. (c) Cells were stimulated with forskolin for 4 h and then glycerol release was determined. (d) RNAs were prepared and differentiation levels were assessed by the expression of the indicated genes. Expression of each gene was normalized to TBP and then relative to the expression in undifferentiated cells. Values are means ± SEM. P is indicated (n = 7 paired depots). Open bars: chin; black bars: knee.