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. 2015 Feb 19;11(2):e1005013. doi: 10.1371/journal.pgen.1005013

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© 2015 Stein et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — Chromosome and spindle dynamics in oocytes expressing AURKA-EGFP (green) and H2B-mCherry (red) were observed by time-lapse live confocal microscopy. Frames at the indicated stages of meiotic maturation were selected from the original time series (S1–S2 Movies), in which images were acquired every 18 min for 16 h. All images are maximal intensity projections of a confocal z series. A-F: Ago2 ^fl/ADH oocytes; G-L: Ago2 ^ADH oocytes. GV: germinal vesicle intact (A, G), GVBD: germinal vesicle breakdown (B, H), MI: metaphase I (C, I), AI: anaphase I (D, J), TI: telophase I (E, K), MII: metaphase II (F, L). The experiment was performed 3 times using at least 10 oocytes per group. Representative images are shown.