| Vibrio cholerae |
|
| JB804 |
01 El Tor strain C6706, Smr
|
[34] |
| JB3 |
01 El Tor strain N16961, Smr
|
[4] |
| JB58 |
01 El Tor strain N16961 ΔlacZ, Smr
|
[4] |
| JB114 |
JB58 ΔvexM
|
[5] |
| JB116 |
JB58 ΔvexH
|
[5] |
| JB432 |
JB58 ΔvexF
|
[5] |
| JB464 |
JB58 ΔvexD ΔvexF ΔvexH ΔvexK ΔvexM
|
[5] |
| JB485 |
JB58 ΔvexB ΔvexD ΔvexF ΔvexH ΔvexK ΔvexM
|
[5] |
| JB495 |
JB58 ΔvexB
|
[4] |
| JB528 |
JB58 ΔvexK
|
[5] |
| JB692 |
JB58 ΔvexD
|
[4] |
| JB694 |
JB58 ΔvexB ΔvexD
|
[4] |
| JB718 |
JB58 ΔvexR ΔvexD
|
This study |
| XBV218 |
JB58 ΔvexR
|
This study |
| XBV220 |
JB58 ΔvexR ΔvexB ΔvexD ΔvexF ΔvexH ΔvexK ΔvexM
|
This study |
| MKW589 |
ΔcpxR lacZ::cpxP-lacZ
|
[26] |
| DT1458 |
JB58 lacZ::cpxP-lacZ
|
[26] |
| DT1572 |
JB58ΔvexB lacZ::cpxP-lacZ
|
[26] |
| DT1460 |
JB485 lacZ::cpxP-lacZ
|
[26] |
| DT1693 |
JB495 lacZ::cpxP-lacZ
|
This study |
| VA367 |
JB58 ΔvexR ΔcpxR lacZ::cpxP-lacZ
|
This study |
| EC8508 |
C6706 Tn::VC0027(ilvA) |
[33] |
| EC20412 |
C6706 Tn::VC0051(purK) |
[33] |
| EC1769 |
C6706 Tn::VC0052(purE) |
[33] |
| EC23411 |
C6706 Tn::VC0164(vexB) |
[33] |
| EC24273 |
C6706 Tn::VC0374(pgi) |
[33] |
| EC14462 |
C6706 Tn::VC0384(cysJ) |
[33] |
| EC5082 |
C6706 Tn::VC0385(cysI) |
[33] |
| EC9587 |
C6706 Tn::VC0386(cysH) |
[33] |
| EC19978 |
C6706 Tn::VC0537(cysM) |
[33] |
| EC11960 |
C6706 Tn::VC0767(guaB) |
[33] |
| EC4709 |
C6706 Tn::VC0774 |
[33] |
| EC11507 |
C6706 Tn::VC0819(aldA-1) |
[33] |
| EC8862 |
C6706 Tn::VC0923 |
[33] |
| EC11848 |
C6706 Tn::VC0968(cysK) |
[33] |
| EC10232 |
C6706 Tn::VC1061 |
[33] |
| EC5818 |
C6706 Tn::VC1169(trpA) |
[33] |
| EC24412 |
C6706 Tn::VC1170(trpB) |
[33] |
| EC12331 |
C6706 Tn::VC1171(trpC/F) |
[33] |
| EC11883 |
C6706 Tn::VC1172(trpD) |
[33] |
| EC11883 |
C6706 Tn::VC1173(trpG) |
[33] |
| EC11131 |
C6706 Tn::VC1174(trpE) |
[33] |
| EC1872 |
C6706 Tn::VC1579 |
[33] |
| EC389 |
C6706 Tn::VC1732(aroA) |
[33] |
| EC14803 |
C6706 Tn::VC1819(aldA-2) |
[33] |
| EC12803 |
C6706 Tn::VC2013(ptsG) |
[33] |
| EC7541 |
C6706 Tn::VC2092(gltA) |
[33] |
| EC18511 |
C6706 Tn::VC2209(vibF) |
[33] |
| EC10553 |
C6706 Tn::VC2348(deoB) |
[33] |
| EC24541 |
C6706 Tn::VC2362(thrC) |
[33] |
| EC19558 |
C6706 Tn::VC2363(thrB) |
[33] |
| EC13310 |
C6706 Tn::VC2364(thrA) |
[33] |
| EC1335 |
C6706 Tn::VC2558(cysC) |
[33] |
| EC13560 |
C6706 Tn::VC2559(cysN) |
[33] |
| EC21282 |
C6706 Tn::VC2560(cysD) |
[33] |
| EC9914 |
C6706 Tn::VC2649(cysE) |
[33] |
| EC20144 |
C6706 Tn::VCA0013(malP) |
[33] |
| EC2460 |
C6706 Tn::VCA0014(malQ) |
[33] |
| EC4499 |
C6706 Tn::VCA0765(ybjU) |
[33] |
| EC9834 |
C6706 Tn::VCA0886(kbl) |
[33] |
| EC3123 |
C6706 Tn::VCA0896(zwf) |
[33] |
| EC14445 |
C6706 Tn::VCA0987(ppsA) |
[33] |
| EC21873 |
C6706 Tn::VCA1046(mtlD) |
[33] |
| Escherichia coli |
| EC100Dpir+ |
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (SmR) nupG pir+ |
Epicentre |
| SM10λpir
|
thi-1 thr leu tonA lacY supE recA::RP4-2-4-Tc::Mu KmR (λ pirR6K) |
[62] |
| ER2566 |
F- glnV44(AS) galK2(Oc) rpsL704(strR) xylA5 mtl-1 argE3(Oc) thiE1 tfr-3 λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5 |
New England BioLabs |
| Plasmids: |
Description: |
|
| pBAD18 |
Expression plasmid, CbR, pBR322 origin of replication |
[63] |
| pCM10 |
Vector for construction of luxCDABE transcriptional fusions, KmR, ori101 |
[64] |
| pDT1076 |
pCM10 containing the vexR promoter region from N16961 |
This study |
| pDT1146 |
pMMB66EH::vexR
|
This study |
| pDT1777 |
pDT1076 with Cm-mark cassette inserted into the vector, CmR
|
This study |
| pJB703 |
pBAD18::vexR
|
This study |
| pMAL-c2 |
Expression plasmid for fusion of proteins to MBP and cytoplasmic expression, CbR, pBR322 origin of replication |
New England BioLabs |
| pMMB66EH |
Expression plasmid, CbR, oriV/T |
[7] |
| pSC137 |
Vector for transposon mutagenesis of bacteria, CmR, oriR6K |
|
| pMAL-c2::vexR
|
vexR cloned into pMAL-c2 |
This study |
| pTL61T |
Vector for construction of lacZ transcriptional fusions, CbR, oriRK2 |
[12] |
| pWM91 |
Suicide plasmid vector used for allelic exchange, CbR, oriR6K/fl |
[65] |
| pWM91::ΔvexR
|
pWM91::ΔvexR
|
This study |
| pXB233 |
pTL61T containing the vexR promoter region from N16961 |
This study |
| pXB228 |
pTL61T containing the vexEF promoter region |
[26] |
| pXB229 |
pTL61T containing the vexGH promoter region |
[26] |
| pXB230 |
pTL61T containing the vexIJK promoter region |
[26] |
| pXB231 |
pTL61T containing the vexCD promoter region |
[26] |
| pXB232 |
pTL61T containing the vexLM promoter region |
[26] |
| pΔR |
cpxR::Km allelic exchange vector |
[37] |
| pJL1P’Z |
Allelic exchange vector for placing cpxP-lacZ into the V. cholerae genome |
[37] |
| Oligonucleotides: |
DNA sequence (5’–3’):: |
|
| 166b-F-XhoI |
AACTCGAGGCAGAGAAATGTGATGT |
|
| 166b-R-XbaI |
AATCTAGAGCCAAACAGCAGGATCG |
|
| 166c-F-XhoI |
TTCTCGAGGGGTCCGGAGACGTACT |
|
| 166c-R-XbaI |
CGTCTAGAGGAGCTGTTTATCGCCG |
|
| Biotin |
GCGGGAGTCGGCAGCG |
|
| MCS4.VexA.R |
CCGGATCCCATTCTGGTGCGAACTCCAAATTAGTGTTG |
|
| VC0166-SacI-F |
CTGAGCTCAAGGGTTCATATGCA |
|
| VC0166-XbaI-R |
TTTCTAGATTAGTGTTGAGTAATTGCA |
|
| VC0166-F1 |
CAGGATCCACTTTAGCACCGTTACTCAG |
|
| VC0166-F2 |
TCATTGCATCCTGTTTATCGCCGTACACTATTTC |
|
| VC0166-R1 |
ACCTCGAGTATTGGCCAGTATGACCTTG |
|
| VC0166-R2 |
CGATAAACAGGATGCAATGATTCAAGCCAGTTGG |
|
| VC0166-F-pMAL-SmaI |
GGCCCGGGTTGCAGAGAAATGTGATGTCTGAAATAGTG |
|
| VC0166-R-pMal-EcoR1 |
GGAATTCTTAGTGTTGAGTAATTGCATCC |
|
|
vexR-F1 |
GCGGGAGTCGGCAGCGATAATAATCCGCTCACCGAG |
|
|
vexR-R1 |
GCGGGAGTCGGCAGCGCCCCTGTTTTGCAATACACTTG |
|
|
vexR-F2 |
GCGGGAGTCGGCAGCGTGCAAAACAGGGGGTATTAG |
|
|
vexR-R2 |
GCGGGAGTCGGCAGCGGCCGTACACTATTTCAGACA |
|
| XWL-BRL-F |
CGCAGGGTTTTCCCAGTCACGAC |
|
