Skip to main content
. 2015 Feb 19;10(2):e0117953. doi: 10.1371/journal.pone.0117953

Fig 7. Caspase-4 acts upstream of PARP and caspase-3, but may play downstream of caspase-8.

Fig 7

(A) SH-EP1 cells transfected with control vector (Ctl) or DN-Cas4 expression vector (DN-Cas4) were treated with Fas antibody (100 ng/ml) for 0, 4, 8, or 16 h, and Western blotting was performed with antibodies against cleaved PARP and caspase-3. β-actin was used as a loading control. Results are representative of at least three experiments. (B) In the presence or absence of caspase-8 inhibitor, z-LETD-fmk (30 μM), Fas-induced cell death was examined using crystal violet staining. *P <0.05 and **P <0.01.