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. 2015 Feb 19;10(2):e0118600. doi: 10.1371/journal.pone.0118600

Fig 6. Anti-TNF antibody prevented the effects of IFNγ on infection of astrocytes by T. cruzi.

Fig 6

(A) Primary astrocyte cell cultures were treated or not with IFNγ (10 ng/mL) in the absence or presence of anti-TNF (10 μg/mL), infected using MOI 10:1, and analyzed 4 hours post-infection (p.i.). (B) Graph shows the mean number of parasites per 100 cells. (C) Representative pictures of IFNγ pretreated astrocytes and infected in the absence or presence of anti-TNF. (D) The graph shows the frequencies of T. cruzi-infected cells bearing different numbers of intracellular forms of the parasite. Data are presented as mean ± SEM of triplicates. **, p < 0.01 and ***, p < 0.001 comparing IFNγ-pretreated astrocytes with not-treated (NT) cells; ##, p < 0.01 comparing IFNγ pretreated astrocytes in absence or presence of anti-TNF.