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. 2015 Feb 19;11(2):e1004649. doi: 10.1371/journal.ppat.1004649

Fig 5. PTX3 delays cellular infiltration kinetics during RRV infection in mice.

Fig 5

(A) 6–7 week old C57BL/6 WT and PTX3-/- (n = 4 per group) mice were infected intraperitoneally with 105 PFU RRV. Peritoneal lavage harvested at 6 hpi was characterized and quantified by flow cytometry using the markers as described in Materials and Methods to determine percentages of neutrophils and inflammatory monocytes. Data are presented as mean ± SEM. ***P < 0.001, Student unpaired t-test. (B) 21-day-old C57BL/6 WT and PTX3 -/- (n = 4–7 per group) mice were infected subcutaneously with 104 PFU RRV. Leukocytes were isolated from the quadriceps harvested at 10 dpi. Cells were characterized and quantified by flow cytometry using the markers as described in Materials and Methods. Total numbers of inflammatory monocytes and NK cells are shown. Data are presented as mean ± SEM. *P < 0.05 **P < 0.005, Student unpaired t-test.