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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Clin Cancer Res. 2014 Dec 5;21(4):925–933. doi: 10.1158/1078-0432.CCR-14-0842

Figure 4. Expression of DNA-PK and related genes in HCC cell lines and inhibition of DNA-PK and ATM activity by specific inhibitors.

Figure 4

DDR gene expression shown as ΔΔCt was determined by quantitative real-time PCR super-arrays relative to HPRT. High levels of DNA-PKcs and partners XRCC5 and XRCC6 were detected (a). Quantification of protein levels for DNA-PK (b) and ATM (c) was by western blotting relative to β-actin and activity was determined using phospho-specific antibodies. DNA-PK or ATM activation following 10 Gy irradiation was inhibited in each of the cell lines in the presence of 1 μM DNA-PK inhibitor NU7441 (b) or 10 μM ATM inhibitor KU55933 (c) respectively. Basal control activity (C) is also shown for comparison. NU7441 (gifted from Celine Cano, Newcastle University), KU55933 (from Marc Hummersone, KuDOS, Cambridge, UK) and doxorubicin were dissolved in dimethyl sulfoxide.