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. 2014 Dec 22;125(8):1292–1301. doi: 10.1182/blood-2014-06-581892

Figure 6.

Figure 6

miR-486-5p cooperates with Gata1s in enhancing growth and self-renewal of FL hematopoietic progenitors. Replating assay of Ter119− hemaopoietic progenitors isolated from E12.5 mouse FL. Cells were transduced with the indicated miR overexpressing or miR down-modulating vectors, cultured in methylcellulose (MC 3231) supplemented with TPO and SCF and replated weekly. miR-125b was used as a positive control of growth-promoting miR.18 (A) Number of colonies in the replating assay of cells isolated from C57BL/6 WT mice. Only miR-125b ectopic expression promoted the growth of progenitor cells. *P < .01 (2-tailed Student t test). (B) Number of colonies in the replating assay of cells isolated from Gata1s KI mice. Both miR-125b and miR-486-5p ectopic expression promoted the growth of progenitor cells. *P < .05 (2-tailed Student t test). (C) qRT-PCR analysis of miR-486-5p expression demonstrating the efficiency of retroviral-mediated miR-486-5p KD in Gata1s KI FL hematopoietic progenitors. (D) Number of colonies in the replating assay of cells isolated from Gata1s KI mice after miR-486-5p KD. The KD of miR-486-5p decreased the rereplating capacity of Gata1s cells. *P < .05 (2-tailed Student t test).