Figure 1.

Wright (Diff Quik) staining of peripheral blood and bone marrow after heat and/or prolonged methanol fixation. Images were captured on an Olympus BX41 microscope using the 100× oil objective, and an Olympus DP72 camera with Olympus CellSens Entry software. (A) Peripheral blood smear heated for 60 minutes at 95°C before staining. (B) Peripheral blood smear fixed in methanol for 30 minutes before staining. (C) Peripheral blood smear heated for 60 minutes at 95°C and then fixed in methanol for 30 minutes before staining. (D) Bone marrow aspirate heated for 60 minutes at 95°C and then fixed in methanol for 30 minutes before staining. Normal erythrocyte morphology and leukocyte details are seen in peripheral blood smears regardless of treatment, and cellular details are preserved in bone marrow aspirate.