Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1973 May;70(5):1419–1422. doi: 10.1073/pnas.70.5.1419

Subunits of Human Chorionic Gonadotropin: Unbalanced Synthesis and Secretion by Clonal Cell Strains Derived from a Bronchogenic Carcinoma

Armen H Tashjian Jr *, Bruce D Weintraub †, Norma J Barowsky *, Alan S Rabson ‡, Saul W Rosen †
PMCID: PMC433510  PMID: 4514312

Abstract

Three clones of ectopic hormone-producing cells from a single human neoplasm (bronchogenic carcinoma) show different rates of synthesis and secretion of chorionic gonadotropin and its alpha and beta subunits. For each clone, the amount of one or the other subunit always exceeded that of the complete hormone molecule. These findings may be analogous to unbalanced immunoglobulin chain synthesis in certain forms of myeloma.

Keywords: ectopic hormones, cell culture, immunoassay

Full text

PDF
1419

Images in this article

1421Image
on p.1421
1421Image
on p.1421

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bancroft F. C., Levine L., Tashjian A. H., Jr Control of growth hormone production by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone. J Cell Biol. 1969 Dec;43(3):432–441. doi: 10.1083/jcb.43.3.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braunstein G. D., Vaitukaitis J. L., Ross G. T. The in vivo behavior of human chorionic gonadotropin after dissociation into subunits. Endocrinology. 1972 Oct;91(4):1030–1036. doi: 10.1210/endo-91-4-1030. [DOI] [PubMed] [Google Scholar]
  3. Gaspard U., Franchimont P. Variations sécrétoires des hormones polypeptidiques placentaires humaines en culture de tissu. C R Acad Sci Hebd Seances Acad Sci D. 1972 Oct 9;275(15):1661–1663. [PubMed] [Google Scholar]
  4. Habener J. F., Powell D., Murray T. M., Mayer G. P., Potts J. T., Jr Parathyroid hormone: secretion and metabolism in vivo. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2986–2991. doi: 10.1073/pnas.68.12.2986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Liddle G. W., Nicholson W. E., Island D. P., Orth D. N., Abe K., Lowder S. C. Clinical and laboratory studies of ectopic humoral syndromes. Recent Prog Horm Res. 1969;25:283–314. doi: 10.1016/b978-0-12-571125-8.50009-0. [DOI] [PubMed] [Google Scholar]
  7. Lipsett M. B. Humoral syndromes associated with cancer. Cancer Res. 1965 Aug;25(7):1068–1073. [PubMed] [Google Scholar]
  8. Pierce J. G. Eli Lilly lecture. The subunits of pituitary thyrotropin--their relationship to other glycoprotein hormones. Endocrinology. 1971 Dec;89(6):1331–1344. doi: 10.1210/endo-89-6-1331. [DOI] [PubMed] [Google Scholar]
  9. Richardson U. I., Tashjian A. H., Jr, Levine L. Establishment of a clonal strain of hepatoma cells which secrete albumin. J Cell Biol. 1969 Jan;40(1):236–247. doi: 10.1083/jcb.40.1.236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Riggs B. L., Arnaud C. D., Reynolds J. C., Smith L. H. Immunologic differentiation of primary hyperparathyroidism from hyperparathyroidism due to nonparathyroid cancer. J Clin Invest. 1971 Oct;50(10):2079–2083. doi: 10.1172/JCI106701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roof B. S., Carpenter B., Fink D. J., Gordan G. S. Some thoughts on the nature of ectopic parathyroid hormones. Am J Med. 1971 May;50(5):686–691. doi: 10.1016/0002-9343(71)90124-0. [DOI] [PubMed] [Google Scholar]
  12. Rosen S. W., Weintraub B. D. Monotropic increase of serum FSH correlated with low sperm count in young men with idiopathic oligospermia and aspermia. J Clin Endocrinol Metab. 1971 Mar;32(3):410–416. doi: 10.1210/jcem-32-3-410. [DOI] [PubMed] [Google Scholar]
  13. Shapiro M., Nicholson W. E., Orth D. N., Mitchell W. M., Liddle G. W. Differences between ectopic MSH and pituitary MSH. J Clin Endocrinol Metab. 1971 Sep;33(3):377–381. doi: 10.1210/jcem-33-3-377. [DOI] [PubMed] [Google Scholar]
  14. Sonnenschein C., Richardson U. I., Tashjian A. H., Jr Chromosomal analysis, organ-specific function and appearance of six clonal strains of rat pituitary tumor cells. Exp Cell Res. 1970 Jul;61(1):121–128. doi: 10.1016/0014-4827(70)90264-8. [DOI] [PubMed] [Google Scholar]
  15. Steiner D. F., Clark J. L., Nolan C., Rubenstein A. H., Margoliash E., Aten B., Oyer P. E. Proinsulin and the biosynthesis of insulin. Recent Prog Horm Res. 1969;25:207–282. doi: 10.1016/b978-0-12-571125-8.50008-9. [DOI] [PubMed] [Google Scholar]
  16. Tashjian A. H., Jr, Yasumura Y., Levine L., Sato G. H., Parker M. L. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology. 1968 Feb;82(2):342–352. doi: 10.1210/endo-82-2-342. [DOI] [PubMed] [Google Scholar]
  17. Vaitukaitis J. L., Braunstein G. D., Ross G. T. A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone. Am J Obstet Gynecol. 1972 Jul 15;113(6):751–758. doi: 10.1016/0002-9378(72)90553-4. [DOI] [PubMed] [Google Scholar]
  18. Vaitukaitis J., Robbins J. B., Nieschlag E., Ross G. T. A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab. 1971 Dec;33(6):988–991. doi: 10.1210/jcem-33-6-988. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES