(A) BAF-6xHis was mixed with E.coli lysates containing recombinant Ankle1, Ankle1ΔCT or an N-terminal Emerin fragment EmerinΔTM and precipitated usining Ni-beads. BAF-bound proteins were detected by Western blotting using antiserum ‘136′ (Ankle1), India His-probe (BAF) and monoclonal antibody ‘MANEM5’ to emerin (gift of Glenn Morris). S, supernatant fraction (10% of input); P, precipitated fraction (50% of preciptated proteins). Molecular weights are indicated in kDa. The scheme shows the Ankle1 constructs used in the assays. (B) Recombinant 6xHis-V5 tagged Ankle1, Ankle1b or LAP2α were incubated with bacterial lysates containing untagged human BAF. His-tagged prey proteins were precipitated using Ni-beads and bound BAF was detected by Western blotting using antibodies to V5 tag and BAF. S, supernatant fraction (10% of input); P, precipitated fraction (50% of preciptated proteins). Note: BAF forms various homomeric complexes even under denaturating conditions (arrows indicating monomeric, dimeric and oligomeric states).