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. 2014 Dec 22;290(8):4561–4572. doi: 10.1074/jbc.M114.573956

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FIGURE 3. — SMLM image of Ca_Vβ₂-N3-YFP expressed in HeLa cells. A, wide field SMLM image of HeLa cells with the focus around the midsection of the cell. F-actin was labeled with Alexa Fluor® 647 phalloidin (green). Ca_Vβ₂-N3 was visualized via a fused YFP (magenta). The overlay of F-actin and Ca_Vβ₂-N3-YFP is shown in white. B and C, 4 × 4-μm subsection indicated by the yellow squares in A. The right panels show the projections of the single molecule localizations delineated by the yellow lines indicated by arrows in B and C. Green lines represent localizations from F-actin, and magenta lines represent localizations from Ca_Vβ₂-N3-YFP. The scale bar for A represents 4 μm. The scale bars for B and C represent 1 μm. Norm., normalized.