TABLE 1.

Parameters describing the distribution of the CFP fluorescence lifetimes in cells expressing various combinations of donor and acceptor constructs

The values were obtained from FLIM-FRET recordings of cells expressing the donor/acceptor combinations indicated. Mean, maximum, and minimum values were calculated over all values for each condition.

	Mean	S.E.	Maximum	Minimum	n
CaVβ2-N3-CFP	2.66	0.01	2.77	2.51	79
CaVβ2-N3-CFP + cytochalasin D	2.68	0.01	2.85	2.46	132
CaVβ2-N3-CFP + YFP-actin	2.05	0.03	2.71	1.57	86
CaVβ2-N3-CFP + YFP-actin + cytochalasin D	2.60	0.02	2.80	1.84	117
CaVβ2-N3-CFP + YFP	2.69	0.01	2.46	2.84	89
CFP	2.69	0.01	2.93	2.49	118
CFP + YFP-actin	2.68	0.01	2.92	2.43	102