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. 2014 Dec 23;290(8):4663–4676. doi: 10.1074/jbc.M114.586883

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FIGURE 8. — Targeted siRNA-mediated gene silencing by homogenous transmural atrial gene transfer. a, schematic representation of the recombinant lentiviral constructs containing green fluorescence protein (GFP) as the reporter gene and four shRNA directed against MLC2 (LV-MLC2) and scrambled sequence (LV-Scramble). b, isolation of GFP-positive atrial myocytes using fluorescence-activated cell sorting. Isotype-matched antibodies were used to control for background fluorescence (FL) and is shown in the left panel labeled Background FL compared with the Cell-Specific FL shown in the right panel.