TABLE 2.

Analysis of NBL1 mutants via luciferase reporter assay

Luciferase reporter assay was performed with 1 nm BMP2.

NBL1 mutant	IC50	Log[IC50(m)] ± S.E.a	Significanceb	Reductionc	Increased
	nm			fold
WT	177	−6.75 ± 0.17		1.00	1.00
ΔC	159	−6.80 ± 0.12	NSe	0.66	1.52
W34A	3900	−5.41 ± 0.60	Yesf	4.16	0.24
Y66A	650	−6.32 ± 0.21	Yesg	3.42	0.29
L60A	160	−6.94 ± 0.10	NS	0.84	1.19
L79A	170	−6.84 ± 0.10	NS	0.89	1.12
A58F	70	−7.20 ± 0.12	Yesf	0.37	2.71
S67Y	22	−7.79 ± 0.08	Yesf	0.12	8.64
A58F/S67Y	27	−7.49 ± 0.07	Yesf	0.14	7.04

^a S.E., standard error of the mean. Experiments were performed in triplicate.

^b Significance measured using Student's t test against WT.

^c Represents fold decrease in BMP2 inhibition (WT/mutant).

^d Represents fold increase in BMP2 inhibition (mutant/WT).

^e NS, not significant.

^f p value of 0.05.

^g p value of 0.1.