TABLE 3.
Comparison of NBL1, NBL1, and PRDC for BMP2, BMP7, and GDF5 inhibition
Luciferase reporter assay was performed with 1 nm BMP2, 3.2 nm BMP7, or 3.7 nm GDF5.
| Protein | IC50 | Log[IC50(m)] ± S.E.a | Significanceb | Fold/PRDCc |
|---|---|---|---|---|
| nm | ||||
| BMP2 | ||||
| PRDC | 0.5 | −9.00 ± 0.05 | 1.0 | |
| NBL1 | 177 | −6.75 ± 0.17 | Yesd | 380.0 |
| NBL1 S67Y | 16 | −7.79 ± 0.08 | Yesd | 32.0 |
| BMP7 | ||||
| PRDC | 18 | −7.76 ± 0.08 | 1.0 | |
| NBL1 | 199 | −6.70 ± 0.14 | Yesd | 11.1 |
| NBL1 S67Y | 23 | −7.65 ± 0.10 | NSe | 1.3 |
| GDF5 | ||||
| PRDC | 92 | −7.04 ± 0.15 | 1 | |
| NBL1 | >10,000 | NCf | NC | NC |
| NBL1 S67Y | >10,000 | NC | NC | NC |
a S.E., standard error of the mean. Experiments were performed in triplicate.
b Significance measured using Student's t-test against PRDC.
c Fold ratio over PRDC inhibition (protein/PRDC).
d p value of 0.05.
e NS, not significant.
f NC, not calculable.