TABLE 1.
Detected modified peptides of TET1
(ph), phosphorylated; (o), O-GlcNAcylated; (ox), oxidized. Localization probability was calculated with MaxQuant software (44). Residue numbering refers to the murine protein sequences specified in supplemental Data S1. The arithmetic mean ± S.D. of the occupancy is depicted for each data set. ND, not detected.
| Modified amino acid | Localization probability | Modified sequence | Mean TET1 | Mean TET1 + OGTmut | Mean TET1 + OGT |
|---|---|---|---|---|---|
| 160 | 1.00 | H … ATVS(ph)PGTENGEQNR | 0.15 ± ND | 0.98 ± ND | 0.34 ± 0.08 |
| 177 | 1.00 | CLVEGES(ph)QEITQSCPVFEER | 0.63 ± 0.01 | 0.32 ± 0.15 | 0.48 ± ND |
| 253 | 0.85 | NT(o)SNQLADLSSQVESIK | ND | 0.07 ± 0.01 | 0.37 ± ND |
| 270 | 0.84 | LS(o)DPSPNPTGSDHNGFPDSSFR | ND | 0.08 ± 0.02 | 0.67 ± 0.06 |
| 320 | 1.00 | FILAGS(ph)QPDVFDTKPQEK | 0.60 ± 0.16 | 0.44 ± 0.03 | 0.30 ± 0.20 |
| 327 | 1.00 | FILAGSQPDVFDT(o)KPQEKa | 0.32 ± 0.47 | 0.36 ± 0.15 | 0.55 ± ND |
| 556 | 0.81 | A … STSS(ph)PPCNSTPPMVER | 0.23 ± 0.10 | ND | ND |
| 561 | 0.89 | A … STSSPPCNS(ph)TPPM(ox)VER | 0.20 ± 0.09 | 0.88 ± ND | ND |
| 734 | 0.98 | QQTNPS(ph)PTFAQTIR | 0.44 ± ND | 0.46 ± 0.33 | 0.32 ± ND |
| 736 | 0.96 | QQTNPSPT(ph)FAQTIR | 0.67 ± 0.06 | ND | ND |
| 794 | 0.77 | DAM(ox)SVTTS(o)GGECDHLK | ND | 0.48 ± ND | 1.00 ± 0.00 |
| 854 | 1.00 | DGS(ph)PVQPSLLSLMK | 0.73 ± 0.13 | 0.54 ± 0.07 | 0.25 ± 0.34 |
| 892 | 0.70 | L … SESSS(ph)PSKPEK | 0.51 ± 0.48 | 0.27 ± 0.03 | 0.79 ± ND |
| 950 | 1.00 | S(ph)PDSFATNQALIKb | 0.68 ± 0.26 | 0.72 ± 0.20 | 0.49 ± 0.11 |
| 969 | 0.74 | SQGYPSS(ph)PT … | 0.61 ± 0.03 | ND | ND |
| 1327 | 0.66 | REAQT(o)SSN … Ka | 0.01 ± 0.00 | ND | 0.79 ± ND |
| 1964 | 0.89 | ELHATTSLRS(ph)PK | 0.33 ± 0.21 | 0.17 ± ND | 0.47 ± 0.33 |
| 2016 | 1.00 | PADRECPDVS(ph)PEANLSHQIPSR | 0.68 ± 0.21 | 0.37 ± 0.18 | 0.81 ± ND |
| 2016 | 0.56 | PADRECPDVS(o)PEANLSHQIPSR | ND | 0.26 ± 0.10 | 0.55 ± 0.41 |
| 2042 | 0.99 | DNVVTVS(ph)PYSLTHVAGPYNR | 0.73 ± 0.12 | ND | 0.38 ± ND |
a Basal O-GlcNAc sites.
b Persistent phosphorylation sites.