TABLE 3.
Detected modified peptides of TET3
(ph), phosphorylated; (o), O-GlcNAcylated; (ox), oxidized. Multiple modifications occurring on one peptide are shown in boldface. Localization probability was calculated with MaxQuant software (44). Residue numbering refers to the murine protein sequences specified in supplemental Data S1. The arithmetic mean ± S.D. of the occupancy is depicted for each data set. ND, not detected.
| Modified amino acid | Localization probability | Modified sequence | Mean TET3 | Mean TET3 + OGTmut | Mean TET3 + OGT |
|---|---|---|---|---|---|
| 360 | 0.93 | VEAPS(o)SS(ph)PAPVPS(ph)PISQR | ND | 0.10 ± 0.07 | 0.91 ± 0.09 |
| 361 | 0.79 | VEAPSS(o)S(ph)PAPVPSPISQRa | 0.02 ± 0.01 | 0.52 ± 0.13 | ND |
| 362 | 1.00 | VEAPSSS(ph)PAPVPSPISQR | 0.03 ± 0.03 | 0.38 ± 0.03 | 0.01 ± ND |
| 362 | 0.67 | VEAPSSS(o)PAPVPSPISQR | ND | 0.96 ± 0.02 | ND |
| 368 | 1.00 | VEAPS(o)SS(ph)PAPVPS(ph)PISQR | 0.34 ± ND | 0.55 ± 0.40 | 0.28 ± ND |
| 478 | 1.00 | S(ph)RDM(ox)QPLFLPVR | 0.46 ± 0.13 | 0.38 ± 0.13 | 0.66 ± ND |
| 557 | 0.83 | S(ph)PSPM(ox)VALQSGST … R | 0.23 ± ND | 0.22 ± 0.27 | ND |
| 557 | 0.76 | S(o)PSPM(ox)VALQSGST … R | ND | ND | 0.44 ± 0.30 |
| 1008 | 0.83 | VS(o)SGAIQVLTAFPR | ND | 0.91 ± 0.01 | 0.36 ± 0.51 |
| 1071 | 0.97 | QEALELAGVT(o)T(o)DPGLSLK | ND | ND | 0.96 ± 0.01 |
| 1072 | 0.89 | QEALELAGVT(o)T(o)DPGLSLK | ND | ND | 0.96 ± 0.01 |
| 1077 | 0.99 | QEALELAGVTT(o)DPGLS(o)LK | ND | 0.50 ± 0.40 | 0.53 ± 0.31 |
| 1105 | 0.89 | YS(o)GNAVVESYSVLGS … R | ND | 0.40 ± 0.07 | 0.73 ± 0.11 |
| 1252 | 0.94 | VPQLHPAS(o)RDPSPFAQSSSCYNR | ND | 0.42 ± ND | 0.95 ± 0.03 |
| 1256 | 0.62 | VPQLHPASRDPS(o)PFAQSSSCYNRa | 0.04 ± 0.01 | ND | 0.98 ± 0.01 |
| 1263 | 0.84 | VPQLHPASRDPSPFAQSSS(o)CYNR | ND | 0.48 ± ND | 0.98 ± 0.03 |
| 1282 | 0.88 | QEPIDPLTQAES(o)IPR | ND | 0.30 ± 0.06 | 0.91 ± 0.09 |
| 1293 | 1.00 | T(o)PLPEAS … SGGPSMSPKa | 0.01 ± 0.00 | 0.53 ± 0.06 | 0.99 ± 0.01 |
| 1318 | 1.00 | TPLPEAS … SGGPSM(ox)S(ph)PK | 0.43 ± 0.07 | 0.00 ± ND | ND |
| 1351 | 0.61 | LNSFGAS(ph)CLTPSHFPES … R | 0.45 ± 0.39 | ND | ND |
| 1404 | 0.76 | FGNGTSALTGPSLT(o)EKa | 0.02 ± 0.02 | ND | 0.74 ± 0.15 |
| 1412 | 1.00 | PWGM(ox)GT(o)GDFNPALK | ND | 0.06 ± 0.00 | 0.65 ± 0.14 |
| 1651 | 0.72 | Q … SAVT(o)VSSYAYTK | ND | ND | 0.18 ± 0.05 |
| 1653 | 0.71 | Q … SAVTVS(o)SYAYTK | ND | 0.43 ± 0.19 | 0.73 ± 0.05 |
| 1654 | 0.77 | Q … SAVTVS(o)S(o)YAYTK | ND | ND | 0.24 ± 0.05 |
| 1658 | 0.99 | G … TDSAVTVSSYAYT(o)Ka | 0.14 ± 0.03 | 0.12 ± 0.05 | 0.75 ± 0.14 |
a Basal O-GlcNAc sites.