FIGURE 2.

Electron transfer between FBD/full-length CPR and cyt c. FBD/CPR (11.3 μm) was reduced to one electron- and two electron-reduced states by stoichiometric titration with sodium dithionite and then rapidly mixed with cyt c in the stopped-flow spectrometer. A, reduction of cyt c (100 μm) by one electron-reduced (blue) and two electron-reduced (red) CPR was monitored at 550 nm. B and C, reduction of cyt c (100 μm) by one electron-reduced (blue) and two electron-reduced (red) FBD was monitored at 550 nm (B), and oxidation of semiquinone FMN was monitored at 630 nm (C). D, dependence of the initial rate of cyt c reduction by one electron-reduced FBD and CPR on varying concentrations of cyt c.