FIGURE 3.
Inhibition of MDR3 ATPase activity by phosphate analogues. A, ATPase activity of purified wild type MDR3 was measured without (w/o, white column) and with 300 μm DOPC lipids (black column) in the presence of orthovanadate, BeFx, and AlFx. The reaction mixture contained 2 mm ATP and inhibitors at a final concentration of 1 or 3 mm. The data are means ± S.D. of at least four independent experiments. B, ATPase activity of Q1174E mutant in the presence of orthovanadate. The data represent the average of at least two independent experiments (mean ± S.D.).