TABLE 3.

Identification of CaMKII phosphorylation sites in r-synGAP

Residues marked with an X are CaMKII phosphorylation sites in r-synGAP identified as described under “Experimental Procedures.” Phosphorylated residues are categorized according to the length of the phosphorylation reaction (2 and 10 min) and detection in MS2 or MS3 spectra. The sites highlighted with an asterisk have phosphorylation false localization rates less than 1% (predicted as described under “Experimental Procedures”).

Residue modified	2 min		10 min
	MS2	MS3	MS2	MS3
Ser-728			X	X
Ser-737	X*	X*	X*	X*
Ser-750			X
Ser-751	X*		X*
Ser-756			X
Ser-764	X
Ser-765	X*	X*	X*	X*
Ser-808	X		X
Ser-809	X		X
Ser-810	X		X
Ser-821	X*		X*
Ser-825	X*	X*	X*	X*
Ser-827	X*	X*	X*	X*
Ser-842	X
Ser-843	X
Ser-882	X*	X*	X*	X*
Ser-883	X*	X*	X*
Thr-885			X*
Ser-892			X*
Thr-897	X*	X*	X*	X*
Thr-898	X	X		X
Ser-990	X	X	X
Ser-1093	X*		X*
Ser-1095	X*		X*
Ser-1099	X*	X*	X*	X*
Ser-1123	X*		X*
Ser-1150	X*	X*	X*
Ser-1171		X	X	X
Ser-1210	X*	X*	X*	X*
Ser-1283	X*	X*	X*	X*
Thr-1291			X*