FIGURE 12.

Cultured aortic VSMCs were treated with lentivirus encoding shOrai3 or control shNT. After Orai3 knockdown, VSMCs were cultured in DMEM/F-12 with 0.4% serum for 24 h prior to treatment with medium containing 10% serum over the time course indicated (30 min to 24 h). VSMCs without serum treatment (time 0 controls) and at different times after serum treatment were lysed or processed for Western blotting using Akt1 (A) and Akt2 (C) isoform-specific antibodies probing phospho-Ser-473 Akt1, total Akt1, phospho-Ser-474 Akt2, Akt2, and β-actin (loading control). B and D represent phospho-Akt (pAkt)/Akt Western blot densitometry performed on VSMCs from three independent shRNA infections. The asterisks indicate statistical significance between the shOrai3 and shNT conditions at the same time point. *, p < 0.05. Error bars represent S.E.