FIGURE 2.

Whole-cell patch clamp electrophysiological recordings were performed on rat synthetic aortic VSMCs that were infected with lentiviruses encoding either control shNT or shLTC₄S. Silencing of LTC₄S significantly inhibited thrombin-activated LRC currents (D) compared with control cells (A). LRC Ca²⁺ and Na⁺ I/V relationships for both conditions are shown in B and E, respectively. Statistics on LRC Ca²⁺ and Na⁺ current densities are shown in C and F, respectively. Ca²⁺ concentration in the pipette solution was buffered to 150 nm using the chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid as described under “Experimental Procedures.” Error bars represent S.E. I/V curves were taken where indicated by the + and * symbols. pF, picofarad.