FIGURE 6.

A, acutely isolated VSMCs from control carotids (right carotids; R) and balloon-injured carotids (left carotids; L) transduced with lentiviral particles encoding either shNT or shOrai3 were harvested and lysed at 2 weeks postinjury, and Orai3 protein levels were determined by Western blotting. In the injured vessel treated with shOrai3, the increase in Orai3 expression induced by balloon injury was significantly attenuated. B–E, PCNA expression in carotid artery was up-regulated after balloon injury. The expression level was not significantly changed upon either LTC₄S (B and C) or Orai3 (D and E) in vivo knockdown. Statistical analysis on PCNA densitometry from six independent rats per group is shown for LTC₄S (C) and Orai3 (E) in vivo knockdown. Error bars represent S.E.