FIGURE 8.

A and B, upon infection of cultured aortic VSMCs with lentivirus encoding either shNT or shLTC₄S, scratch wound migration assays were performed in the presence of 10% FBS. The area of migrated cells at 12 h after scratch in control shNT-infected and shLTC₄S-infected VSMCs (as seen in representative images in A) was determined and analyzed from three independent experiments (4 wells/condition) using NIH ImageJ software (B). C, statistical analysis on parallel Western blotting experiments documenting LTC₄S protein knockdown in VSMCs used in migration assays from three independent experiments. *, p < 0.05; **, p < 0.01. Error bars represent S.E.