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. 2014 Dec 24;290(8):5015–5027. doi: 10.1074/jbc.M114.625822

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FIGURE 9. — A and B, scratch wound migration assay using cultured VSMCs in the presence of 10% FBS. Upon Orai3 knockdown by infection with lentivirus encoding shOrai3, the migrated area at 12 h after scratch in control shNT-infected and shOrai3-infected VSMCs (A) was determined and analyzed from three independent experiments (4 wells/condition) using NIH ImageJ software (B). C, representative anti-Orai3 Western blotting experiment documenting Orai3 protein knockdown in VSMCs and statistical analysis on three Western blotting experiments using cells assayed in migration assays. *, p < 0.05; ***, p < 0.001. Error bars represent S.E.