FIGURE 5.

Retb is produced by photoreceptors but not dependent on the presence of OSs. A, IF images of WT retinal sections co-labeled with Retb (red) and arrestin (green) (left panels) or Retb alone (right panels) in retinas taken from dark-adapted (top panels) and light-adapted (bottom panels) mice. B, immunoblot detection of Retb protein in P30 wild-type and Rd/Rd neural retinal extracts. No Retb was detected in any of the four different Rd/Rd retinal extracts. C, immunoblot of extracts from wild-type and Rho^−/− neural retinas at P21, probed with anti-Retb antibody. Retb is detected in neural retinal extracts from both Rho^−/− and wild-type animals. D, IF of Retb (red) in P21 Rho^−/− retina (left panel), for Rds (middle panel) or ezrin (right panel) in green, and nuclei counterstained with DAPI. Scale bars, 10 μm. OS, outer segment; IS, inner segment; ONL, outer nuclear layer.