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. 2014 Dec 30;290(8):5065–5077. doi: 10.1074/jbc.M114.603217

FIGURE 5.

FIGURE 5.

NLRP3−/− mice exhibit increased MMP-9 activity and decreased Bcl-2 expression. Lung samples were isolated from WT and NLRP3−/− mice exposed to normoxia or hyperoxia for 72 h. A, Mmp9 mRNA levels in lung tissues were assessed by real-time RT-PCR analysis (n = 4–6 for each in normoxia, and n = 7–8 for each in 72-h hyperoxia). B, MMP-9 expression was assessed in the lungs (n = 3–4 for each, 72-h hyperoxia). C, Bax and Bcl2 mRNA expression in the lungs was assessed by real-time RT-PCR analysis (n = 4–6 for each in normoxia, and n = 6–13 for each in 72-h hyperoxia). D and E, Western blot analysis of Bax and Bcl-2 in the lungs (n = 5–6 for each in 72-h hyperoxia). Data are expressed as the means ± S.E. *, p < 0.05; **, p < 0.01.