FIGURE 9.

Role of neutrophils and macrophages in the response of NLRP3^−/− mice to hyperoxia. A, the migration of WT and NLRP3^−/− neutrophils in response to CXCL1 and the supernatants from cultures of MLE 12 cells was assessed using a Transwell migration assay (n = 3 for each). Nil, no treatment. B, total RNA was extracted from immortalized murine WT and NLRP3^−/− BMDMs exposed to normoxia or hyperoxia for 48 h. Ccl2 and Cxcl1 mRNA expression was assessed by real-time RT-PCR analysis (n = 3–4 for each). C, adoptive transfer of WT or NLRP3^−/− neutrophils into NLRP3^−/− mice was performed 5 h before exposure to hyperoxia. The survival of NLRP3^−/− mice administered WT or NLRP3^−/− neutrophils was analyzed using the Kaplan-Meier method (n = 9–15 for each). Data are expressed as the means ± S.E. *, p < 0.05; **, p < 0.01.