TABLE 1.
Identified gC-1 N- and O-glycopeptides
The figures where the significant ions are found in the corresponding mass spectra are shown in the last column.
| Residues | Peptide sequence | Glycan composition | Measured mass [M+H]+ | Theoretical mass [M+H]+ | Δ Mass | Figure |
|---|---|---|---|---|---|---|
| ppm | ||||||
| 76–95 | TTPTEPASPPTTPKPTSTPK | 2036.0573 | 2036.0546 | 1.3 | Supplemental Fig. S1A | |
| HexNAc1 | 2239.1354 | 2239.1340 | 0.6 | Fig. 7A, supplemental Figs. S1, A and B, and S3A | ||
| HexNAc2 | 2442.2159 | 2442.2134 | 1.0 | Fig. 7, A and B, supplemental Figs. S1, A and C, and S3B | ||
| HexNAc3 | 2645.2964 | 2645.2928 | 1.4 | Fig. 7A, supplemental Figs. S1A and D, and S3C | ||
| HexNAc4 | 2848.3766 | 2848.3722 | 1.5 | Fig. 7A, supplemental Figs. S1, A and E, and S3D | ||
| HexNAc5 | 3051.4574 | 3051.4516 | 1.9 | Fig. 7A, supplemental Figs. S1, A and F, and S3E | ||
| HexNAc6 | 3254.5376 | 3254.5310 | 2.0 | Fig. 7A, supplemental Figs. S1, A and G, and S3F | ||
| HexNAc7 | 3457.6103 | 3457.6104 | 0.0 | Fig. 7A, supplemental Fig. S1A | ||
| Hex1HexNAc2 | 2604.2693 | 2604.2662 | 1.2 | Fig. 7A, supplemental Fig. S2A | ||
| Hex1HexNAc3 | 2807.3444 | 2807.3456 | −0.4 | Fig. 7A, supplemental Fig. S2A | ||
| Hex1HexNAc4 | 3010.4342 | 3010.4250 | 3.1 | Fig. 7A, supplemental Fig. S2A | ||
| Hex1HexNAc5 | 3213.5111 | 3213.5044 | 2.1 | Fig. 7A, supplemental Fig. S2A | ||
| Hex1HexNAc6 | 3416.5781 | 3416.5838 | 1.7 | Fig. 7A, supplemental Fig. S2A | ||
| Hex4HexNAc4 | 3496.5851 | 3496.5834 | 0.5 | Supplemental Fig. S2B | ||
| Hex5HexNAc5 | 3861.7220 | 3861.7156 | 1.7 | Supplemental Fig. S2B | ||
| Hex6HexNAc6 | 4226.8361 | 4226.8478 | −2.8 | Supplemental Fig. S2B | ||
| Neu5Ac1Hex6HexNAc6 | 4517.9469 | 4517.9432 | 0.8 | Supplemental Fig. S2B | ||
| 96–105 | SPPTSTPDPK | 1026.5109 | 1026.5102 | 0.7 | ||
| HexNAc1 | 1229.5903 | 1229.5896 | 0.6 | Supplemental Figs. S7, A and B, and S8D | ||
| HexNAc2 | 1432.6703 | 1432.6690 | 0.9 | Supplemental Figs. S7, A and 7C, and S8C | ||
| HexNAc3 | 1635.7493 | 1635.7484 | 0.6 | Supplemental Figs. S7, A and D, and S8B | ||
| HexNAc4 | 1838.8293 | 1838.8278 | 0.8 | Supplemental Figs. S7, A and E, and S8A | ||
| 96–107 | SPPTSTPDPKPK | 1251.6584 | 1251.6579 | 0.4 | ||
| HexNAc1 | 1454.7386 | 1454.7373 | 0.9 | Supplemental Figs. S4, A and B, and S6E | ||
| HexNAc2 | 1657.8176 | 1657.8167 | 0.5 | Supplemental Figs. S4, A and C, and S6D | ||
| HexNAc3 | 1860.8975 | 1860.8961 | 0.8 | Supplemental Figs. S4, A and D, and S6C | ||
| HexNAc4 | 2063.9771 | 2063.9755 | 0.8 | Supplemental Figs. S4, A and E, and S6B | ||
| Hex3HexNAc3 | 2347.0580 | 2347.0545 | 1.5 | Supplemental Figs. S5, A and C, and S6A | ||
| Hex1HexNAc4 | 2226.0359 | 2226.0283 | 3.4 | Supplemental Fig. S4A | ||
| Hex2HexNAc4 | 2388.0803 | 2388.0811 | −0.3 | Supplemental Fig. S5, A and B | ||
| Hex3HexNAc4 | 2550.1349 | 2550.1339 | 0.4 | Supplemental Fig. S5, A and D | ||
| Hex4HexNAc4 | 2712.1895 | 2712.1867 | 1.0 | Supplemental Fig. S5, A and E | ||
| Neu5Ac1Hex3HexNAc3 | 2638.1519 | 2638.1499 | 0.8 | Supplemental Fig. S5A | ||
| Neu5Ac1Hex3HexNAc4 | 2841.2333 | 2841.2293 | 1.4 | Supplemental Fig. S5A | ||
| Neu5Ac1Hex4HexNAc4 | 3003.2879 | 3003.2821 | 1.9 | Supplemental Fig. S5A | ||
| 106–114 | PKNNTTPAK | dHex1Hex3HexNAc4 | 2415.0680 | 2415.0655 | 1.0 | Supplemental Fig. S9A |
| dHex1Hex5HexNAc4 | 2739.1748 | 2739.1711 | 1.4 | Supplemental Figs. S9, A and B | ||
| dHex1Hex6HexNAc5 | 3104.3132 | 3104.3033 | 3.2 | Supplemental Fig. S9A | ||
| dHex1Hex7HexNAc6 | 3469.4369 | 3469.4355 | 0.4 | Supplemental Fig. S9A | ||
| dHex1Hex8HexNAc7 | 3834.5744 | 3834.5677 | 1.7 | Supplemental Fig. S9A | ||
| 108–114 | NNTTPAK | dHex1Hex3HexNAc4 | 2189.9361 | 2189.9178 | 8.4 | Supplemental Fig. S10A |
| dHex1Hex5HexNAc4 | 2514.0269 | 2514.0234 | 1.4 | Supplemental Figs. S10, A and C, and S11A | ||
| dHex1Hex6HexNAc5 | 2879.1602 | 2879.1556 | 1.6 | Supplemental Figs. S10 A and E, and S11B | ||
| dHex1Hex7HexNAc6 | 3244.2950 | 3244.2878 | 2.2 | Supplemental Fig. S10A | ||
| dHex1Hex8HexNAc7 | 3609.4256 | 3609.4200 | 1.6 | Supplemental Fig. S10A | ||
| Neu5Ac1dHex1Hex5HexNAc4 | 2805.1229 | 2805.1188 | 1.5 | Supplemental Fig. S10, B and D | ||
| Neu5Ac2dHex1Hex5HexNAc4 | 3096.2195 | 3096.2142 | 1.7 | Supplemental Fig. S10B | ||
| Neu5Ac1dHex1Hex6HexNAc5 | 3170.2598 | 3170.2510 | 2.8 | Supplemental Fig. S10, B and F | ||
| Neu5Ac2dHex1Hex6HexNAc5 | 3461.3546 | 3461.3464 | 2.4 | Supplemental Fig. S10B | ||
| Neu5Ac1dHex1Hex7HexNAc6 | 3535.3886 | 2525.3832 | 1.5 | Supplemental Fig. S10B | ||
| 146–151 | FRNSTR | dHex1Hex4HexNAc3 | 2183.9207 | 2183.9184 | 1.1 | Supplemental Fig. S12, A and C |
| dHex1Hex5HexNAc3 | 2345.9744 | 2345.9712 | 1.4 | Supplemental Figs. S12, A and E, and S13A | ||
| dHex1Hex5HexNAc4 | 2549.0531 | 2549.0506 | 1.0 | Supplemental Figs. S12. A and D, and S13C | ||
| dHex1Hex6HexNAc5 | 2914.1876 | 2914.1828 | 1.6 | Supplemental Figs. S12A and S13D | ||
| dHex1Hex7HexNAc6 | 3279.3215 | 3279.3150 | 2.0 | Supplemental Fig. S12A | ||
| dHex1Hex8HexNAc7 | 3644.4602 | 3644.4472 | 3.6 | Supplemental Fig. S12A | ||
| 361–372 | RNATGLALVLPR | Hex5HexNAc4 | 2903.3588 | 2903.3613 | −0.9 | Supplemental Fig. S14, A–C |
| dHex1Hex5HexNAc4 | 3049.4189 | 3049.4192 | −0.1 | Supplemental Fig. S14A | ||
| dHex2Hex5HexNAc4 | 3195.4790 | 3195.4771 | 0.6 | Supplemental Fig. S14A | ||
| Neu5Ac1dHex1Hex5HexNAc4 | 3340.5113 | 3340.5146 | −1.0 | Supplemental Figs. S14, A and D |