FIGURE 5.

shRNA knockdown of endogenous I2-containing SAP102. A, HEK-293 cells were co-transfected with SAP102 or SAP102ΔI2 and scrambled shRNA or SAP102 I2 shRNA, and the immunoblots of cell lysate were probed with pan-SAP102 or tubulin antibody. Data represent the means ± S.E. (n = 3 independent experiments; *, p < 0.05, Student's t test). B, primary cortical neurons (DIV4) were infected with lentivirus containing SAP102 I2 shRNA or scrambled shRNA. The cell lysates were prepared at DIV14, resolved by SDS-PAGE, and immunoblotted with I2-specific SAP102 or tubulin antibody. Data represent the means ± S.E. (n = 3 independent experiments; *, p < 0.05, Student's t test).