FIGURE 6.

KYP-2047 alters the conformational forms of active PREP but not PREP(S554A). Recombinant PREP and PREP(S554A) proteins on native-PAGE revealed three different conformations that likely represent different open/close monomeric and oligomeric forms of the protein. However, inhibition of PREP with KYP-2047 shifts the slower migrating conformations into a single band, likely representing closed monomeric conformation of PREP. PREP(S554A) had similarly three conformations, but they were not affected by KYP-2047.