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. 2015 Jan 2;290(8):5127–5140. doi: 10.1074/jbc.M114.599266

FIGURE 4.

FIGURE 4.

Anti-FcγRIIIa/b antibodies inhibit AHG binding. A, increasing amount of Alexa Fluor-488-AHG from 0.5, 1, 2.5, and 5 μg show linear increase in AHG-binding cells (1.61, 11.20, 29.68, and 66.47%) and increase in MFI (16.96, 56.90, 99.34, and 193.98). B, monoclonal anti-FcγRIIIa/b antibody at 5 μg inhibited the AHG binding. Isotype control 10 μg protein did not inhibit AHG binding (C). Polyclonal anti-FcγRIIIb at 5 μg inhibits the AHG binding (red) (D). Polyclonal anti-FcγRI showed slight inhibition (E). Unstained cells (red) and cells stained with AHG (blue) (F). Goat F(ab)2 fragment did not inhibit the AHG binding (G). Histogram unstained cells (H), stained with AHG (I), and overlay scatter of H and I (J). Unstained cells (K) and isotype control (L). Data are representative of two independent experiments.